Technical Data
H7023-12D
Hox B4 (Homeobox Protein B4, HOXB4, HOX-B4, Homeobox 2F, Hox 2F, HOX 2, Hox 2.6)
Description:
HOX homeobox genes play a role both in the early stem cell function as well as in later stages of hematopoietic differentiation. HOXB4, specifically, is a homeotic selector gene implicated in self-renewal of definitive HSCs. Expression of HOXB4 in primitive progenitors combined with culture on hematopoietic stroma induced a switch to the definitive HSC phenotype. Activation of WNT signaling in HSCs induced increased expression of HOXB4. Perturbations of HOX gene expression can cause leukemia.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Immunohistochemistry (formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa100-150 of human HOXB4.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human HOXB4. Species Crossreactivity: feline, chimpanzee, bovine, mouse, rat. Species sequence homology: chicken, gorilla, equine, opossum, porcine, rhesus monkey, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kyba, M. et al. Cell. 109:29-37 (2002). 2. Reya, T. et al. Nature. 423:409-414 (2003).