		Technical Data
H7023-15W	Hlxb9, CT (Homeodomain Protein Hb9, Motor Neuron and Pancreas Homeobox 1, Novel Protein Similar to Vertebrate Homeo Box HB9 (HLXB9), mnx1, Dr.91917, hb9, CH211-266O5.2-001)

Description:
Motor neuron and pancreas homeobox 1 (mnx-1), previously known as Hb9, is a member of the mnx gene family, of which the mouse transcription factor Hb9 is a well-known member. Mouse Hb9 is necessary to the development of the pancreas, in initial morphogenesis and subsequent differentiation of beta cells. Zebrafish expression of mnx genes occurs in all three germ layers. Knockdown studies show that zebrafish mnx is necessary to the differentiation of beta cells but not the early morphogenesis of the pancreas. Mnx-1 is one of many genes involved in motor neuron regeneration in zebrafish.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Optimal dilutions to be determined by the researcher.

Control Peptide: H7023-15Y

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Zebrafish	Rabbit

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to the C-terminal region of zebrafish Mnx1.

Purity:

Purified by immunoaffinity chromatography.

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

Specificity:

Recognizes zebrafish Mnx-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wendik, B et al (2004) Dev. Biol. 268(2):372-383. 2. Reimer, MM et al (2008) J. Neurosci. 28(34):8510-8516.