Technical Data
H7980-36
Hyaluronic Acid Binding Protein 1, Recombinant, Mouse (Hyaluronan Binding Protein 1, HABP1, C1qBP, C1q-R, Human complement component 1, q subcomponent binding protein)
50ug
Molecular Biology Storage: -20°CShipping: Blue Ice
Hyaluronan binding protein 1 (HABP1), also known as C1qBP/C1qR and p32, is a ubiquitous acidic glycoprotein that functions in spermatogenesis and as a receptor for proinflammatory molecules (1, 2). HABP1 is synthesized with a 71 amino acid (aa) N-terminal preproprotein and a 208 aa mature region (3). The 3 kD mature mouse HABP1, which contains a MAM33-like sequence, shares 90% and 99% aa sequence identity with human and rat HABP1, respectively. HABP1 assembles into a doughnut shaped trimer, with negatively charged residues asymmetrically distributed on one face lining the channel of the complex (4). HABP1 can be cleaved by cell surface MMP-14/MT1-MMP, a protease important in angiogenesis and tumor metastasis (5). Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6-9). Within the cell, HABP1 binds to molecules containing the C1q globular domain, multiple isoforms of PKC, mitochondrial Hrk, the cytoplasmic tails of adrenergic and GABA-A receptors, the mRNA splicing factor ASF/SF2, and the CBF transcription factor (10-16). Apoptosis and direct phosphorylation by Erk1/2 induces HABP1 translocation to the nucleus (17).

A DNA sequence encoding the mature mouse HABP1 (Leu 72-Gln 279; Accession # NP_031599), fused with 2X Met at the N-terminus and a six-histidine tag at the C-terminus, was expressed in E. coli.

Calculated molecular mass of ~ 24.8kD.

Activity:
Measured by its ability to bind human C1q.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Source: Recombinant Mouse
Purity: 95%, as determined by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin: 1EU/1ug.
Form: Supplied as a lyophilized powder in PBS. Reconstitute with PBS to 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Thakur, S.C. et al., 2006, J. Androl. 27:604.
2. Peerschke, E.I.B. and B. Ghebrehiwet, 2007, Immunobiology 212:333. 12. Sunayama, J. et al., 1994, Cell Death Differ. 11:771.
3. Lynch, N.J. et al., 1997, FEBS Lett. 418:111. 13. Pupo, A.S. and K.P. Minneman, 2003, J. Recept. Signal Transduct.
4. Jiang, J. et al., 1999, Proc. Natl. Acad. Sci. 96:3572. Res. 23:185.
5. Rozanov, D.V. et al., 2002, J. Biol. Chem. 277:9318. 14. Schaerer, M.T. et al., 2001, J. Biol. Chem. 276:26597.
6. Deb, T.B. and K. Datta, 1996, J. Biol. Chem. 271:2206. 15. Peterson-Mahrt, S.K. et al., 1999, EMBO J. 18:1014.
7. Lim, B.L. et al., 1996, J. Biol. Chem. 271:26739. 16. Chattopadhyay, C. et al., 2004, Nucleic Acids Res. 32:3632.
8. Grebrehiwet, B. et al., 1994, J. Exp. Med. 179:1809. 17. Majumdar, M. et al., 2002, Biochem. Biophys. Res. Commun.
9. Waggoner, S.N. et al., 2007, J. Leukoc. Biol. 82 epub. 291:829.
10. Innamorati, G. et al., 2006, Cell. Signal. 18:761.
11. Robles-Flores, M. et al., 2002, J. Biol. Chem. 277:5247.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.