Technical Data
5-Hydroxytryptamine, Conjugated
Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.

Using a conjugate 5 hydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compounds Cross-reactivity ratio(a)
5 Hydroxytryptamine-G-PL 1
5 Hydroxytryptamine-G-PL (b) 1/10
5 Methoxytryptamine-G-PL 1/25
Tryptamine-G-PL 1/51
5 Hydroxytryptophan-G-PL 1/175
5 Hydroxytryptamine 1/1,150
Tryptophan-G-PL 1/3,400
5 Methoxytryptophan-G-PL 1/19,800

(a) : 5 Hydroxytryptamine-G-PL concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
(b) : Non-reduced conjugate
G = Glutaraldehyde, PL = poly-Lysine

Recommended dilution
The antiserum was tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 5-hydroxytryptamine antibodies stains well between a 1/2,000-1/5,000.

Storage and Stability:
May be stored at 4░C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20░C. Aliquots are stable for at least 12 months at -20░C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ug-20░CBlue IceRabbit
5 Hydroxytryptamine Glutaraldehyde Carriers.
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Recognizes protein-conjugated Hydroxytryptamine,5-. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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