Technical Data
H9807-01
Hypoxia Inducible Factor 1a (HIF-1a)
Description:
Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF hydroxylases provide insight into hypoxic cell responses, which may be used to help isolate therapeutic targets.

Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500 (ECL)
Immunohistochemistry (Paraffin and frozen): 1:25-1:100
Optimal dilutions to be determined by the researcher.

Positive control:
Nuclear extracts

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b3C142Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Fusion protein containing aa432-528 of human HIF-1 alpha. Cellular Localization: Nuclear.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Specificity:
Recognizes human HIF-1 alpha. Recognizes bands at 120kD representing HIF-1 alpha in induced tissues and cells. Multiple bands may be seen at 100-120kD representing post-translational modification of HIF-1 alpha. Species crossreactivity: monkey, sheep, mouse, rat, ferret, porcine, hamster, bovine and rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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JBC Papers in Press. Published on October 18, 2001 as Manuscript M106282200
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