Technical Data
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850) (PE)
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4

Functional Application: Antibody blocks binding of Recombinant GL50-muIg to ICOS

Suitable for use in Flow Cytometry and FLISA. Other applications not tested.

Recommended Dilution
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable at 4C as an undiluted liquid. Dilute only pror to immediate use. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity.
MabIgG17H152Affinity Purified
120Tests4C Do Not FreezeBlue IceHumanMouse
As reported
Human HPB-MLT cells, recombinant human ICOS-muIg
Purified by Protein A affinity chromatography.
Supplied as a liquid in 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3.
Binds to recombinant and cell surface ICOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.
2) Riley, J.L., C.H. June, et al. 2001, J. Immunol.166 : 4943-4948.
3) Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657.
4) Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.