Technical Data
ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)(PE)
Human ICOS Ligand, also called B7-H2, B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20-25% amino acid identity. Human and mouse ICOS Ligand share ~49% amino acid identity. ICOS Ligand has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. Human ICOS Ligand is a 309 amino acid (aa) protein with a putative 18aa signal peptide, a 239aa extracellular domain, an 18aa transmembrane region and a 33aa cytoplasmic domain. Human ICOS Ligand is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 10ul labels 10e5 cells or 50ul of packed whole blood.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C before opening. DO NOT FREEZE! Stable for at least 12 months at 4C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
MabIgG2b8H104Affinity Purified
100Tests4C Do not freezeBlue IceHumanMouse
As reported
Recombinant corresponding to human ICOS Ligand extracellular domain expressed in NS0 cells.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Recognizes human ICOS Ligand.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.