Technical Data
ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein GL50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG, ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7-H2 ICOS Ligand)
CD275, also known as ICOS ligand (inducible T cell co-stimulator ligand). CD275 is a member of the B7 family, and interaction with its receptor CD278 (ICOS) plays an important role in T cell co-stimulation and inflammatory immune reactions.
CD275 is expressed on activated B-cells, monocytes and dendritic cells and is also induced on non-lymphoid tissue cells such as endothelial cells, epithelial cells and muscle cells. CD275 has an important physiological role in the reactivation of effector/memory T cells on the endothelium, thus controlling entry of immune cells into inflamed tissue.
The CD278-CD275 pathway is thought to be critical in the immune response to transplants, allergies and autoimmune diseases.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Positive Control for Immunohistochemistry:

P3U1 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG19A192Affinity Purified
200ug4C/-20C (No Glycerol)Blue IceHumanMouse
CD275-L cells
Purified by Protein G affinity chromatography
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Recognizes human CD275.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Youngnak-Piboonratanakit, P. et al. (2006) Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues. Scand. J. Immunol. 63: 191-198.