Technical Data
I0650-01K
ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein GL50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG, ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7-H2 ICOS Ligand)
Description:
CD275, also known as ICOS ligand (inducible T cell co-stimulator ligand). CD275 is a member of the B7 family, and interaction with its receptor CD278 (ICOS) plays an important role in T cell co-stimulation and inflammatory immune reactions.
CD275 is expressed on activated B-cells, monocytes and dendritic cells and is also induced on non-lymphoid tissue cells such as endothelial cells, epithelial cells and muscle cells. CD275 has an important physiological role in the reactivation of effector/memory T cells on the endothelium, thus controlling entry of immune cells into inflamed tissue.
The CD278-CD275 pathway is thought to be critical in the immune response to transplants, allergies and autoimmune diseases.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Positive Control for Immunohistochemistry:
Tonsil

Hybridoma:
P3U1 myeloma cells with spleen cells from BALB/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG19A192Affinity Purified
SizeStorageShippingSourceHost
200ug4C/-20C (No Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
CD275-L cells
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Specificity:
Recognizes human CD275.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Youngnak-Piboonratanakit, P. et al. (2006) Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues. Scand. J. Immunol. 63: 191-198.