Technical Data
ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein GL50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG, ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7-H2 ICOS Ligand)
CD275, also known as ICOS ligand, is a member of the B7 family, and interaction with its receptor CD278 (ICOS) plays an important role in T cell co-stimulation and inflammatory immune reactions.

CD275 is expressed on activated B-cells, monocytes and dendritic cells and is also induced on non-lymphoid tissue cells such as endothelial cells, epithelial cells and muscle cells. CD275 has an important physiological role in the reactivation of effector/memory T cells on the endothelium, thus controlling entry of immune cells into inflamed tissue.

Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:20-1:200; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

P3U1 myeloma cells with spleen cells from BALB/c mice.

Positive Control Tissue:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG19A192Affinity Purified
100ug-20CBlue IceHumanMouse
CD275-L cells
Purified by Protein G affinity chromatography
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Recognizes human ICOS ligand.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Youngnak-Piboonratanakit, P. et al. (2006) Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues. Scand. J. Immunol. 63: 191-198.