		Technical Data
I0650-01K4	ICOS Ligand (CD275, Inducible T cell Co-stimulator Ligand, B7-H2, B7 Homolog 2, B7-like Protein GL50, B7 Related Protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG, ICOSLG, KIAA0653, LICOS, Transmembrane Protein B7-H2 ICOS Ligand) (PE)

Description:
Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat; 10ul labels 10e6 cells in 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1	9A192	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C/-20°C (No Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

CD275-L cells.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

Specificity:

Recognizes human CD275.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Youngnak-Piboonratanakit, P. et al. (2006) Expression and regulation of human CD275 on endothelial cells in healthy and inflamed mucosal tissues. Scand. J. Immunol. 63: 191-198.