		Technical Data
I0650-01S	ICOS (CD278, Inducible T-cell Costimulator, Activation-inducible Lymphocyte Immunomediatory Molecule, AILIM) (APC)

Description:
Inducible co-stimulator also called AILIM and CRP-1 (CD28-related protein-1), is a member of the CD28 family of immune co-stimulatory receptors. Other family members are CD28, CTLA-4, and PD-1. Human ICOS is a homodimeric type I transmembrane protein consisting of 199aa with a putative 20aa signal sequence, a 121aa extracellular domain, a 23aa transmembrane region, and a 35aa cytoplasmic domain. ICOS shares ~39% aa similarity with CD28 and CTLA-4. Human and mouse ICOS share ~72% aa identity. ICOS is expressed on most CD45RO+ cells. ICOS expression is up-regulated within ~24-48h of activation on T helper (Th) primed cells. B7-H2, a member of the B7 family of co-stimulatory ligands, has been identified as the ICOS ligand. The B7-H2/ ICOS interaction appears to play roles in T cell-dependent B cell activation and Th differentiation.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Excitation: 620-650nm

Emission: 660-670nm

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Type	Isotype	Clone	Grade
Mab	IgG1	12K105	Affinity Purified

Size		Storage	Shipping	Source	Host
100 Tests		4°C Do Not Freeze	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant corresponding to human ICOS expressed in NSO cell line.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with Allophycocyanin (APC).

Specificity:

Recognizes human ICOS.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Aicher, A. et al. (2000) J. Immunol. 164:4689. 2. Coyle, A.J. et al. (2000) Immunity 13:95. 3. Coyle, A.J. & J.C. Gutierrez-Ramos (2001) Nat. Immunol. 2:203. 4. Gonzalo, J.A. et al. (2001) J. Immunol. 166:1. 5. Hutloff, A. et al. (1999) Nature 397:263. 6. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. 7. Yoshinaga, S.K. et al. (1999) Nature 402:827.