		Technical Data
I0650-04	ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)

Description:
B7-H2 is detected on the surface of monocyte derived immature dendritic cells. It is a putative ligand for ICOS (inducible costimulator) from CD28 family of T cell-costimulators. Costimulatory interactions between the B7 family ligands and their receptors like CD28, CTLA-4 and ICOS play critical role in the growth, differentiation and death of T cells. B7-H2 has four conserved structural cysteins in its extracellular domain which are believed to form the Ig V like and IgC-like domain. In addition, B7-H2 has a conserved tyrosine residue in the Ig V-like domain at position of aa80. B7-H2 shares ~46% homology at amino acid level to B7h/B7RP-1, a recently described B7 like protein of mouse origin.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: Tested on BOSC cells transiently transfected with an ICOS Ligand expression vector.
Optimal conditions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6D469	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Vector containing the cDNA of human ICOS Ligand.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.

Specificity:

Recognizes the extracellular domain of human ICOS Ligand native protein on antigen presenting cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

T1. Tumor necrosis factor-alpha regulates the expression of inducible costimulator receptor ligand on CD34(+) progenitor cells during differentiation into antigen presenting cells: G. Richter, et al.; J. Biol. Chem. 276, 45686 (2001)