Technical Data
I0650-04A
ICOSLG (ICOS Ligand, B7 Homolog 2, B7-H2, B7-like Protein Gl50, B7-related Protein 1, B7RP-1, CD275, B7H2, B7RP1, ICOSL, KIAA0653)
Description:
B7-H2 is detected on the surface of monocyte derived immature dendritic cells. It is a putative ligand for ICOS (inducible costimulator) from CD28 family of T cell-costimulators. Costimulatory interactions between the B7 family ligands and their receptors like CD28, CTLA-4 and ICOS play critical role in the growth, differentiation and death of T cells. B7-H2 has four conserved structural cysteins in its extracellular domain which are believed to form the Ig V like and IgC-like domain. In addition, B7-H2 has a conserved tyrosine residue in the Ig V-like domain at position of aa80. B7-H2 shares ~46% homology at aa level to B7h/B7RP-1, a recently described B7 like protein of mouse origin.

Applications:
Suitable for use in Flow Cytometry, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b2D3Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
Recombinant corresponding to human ICOSLG.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human ICOSLG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.