Technical Data
I0800-14S
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10
Western Blot: 1:40 detects a singe band at ~130kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b,k7C204Supernatant
SizeStorageShippingSourceHost
1ml4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant human iNOS
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes human iNOS (25-54) and recombinant human iNOS (Type II). Does not react to human nNOS (201-231), recombinant human nNOS (Type I), human eNOS (25-54) and recombinant human eNOS (Type III). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of human iNOS and was found to bind to a defined region of the human iNOS sequence, residues 25-54.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.