Technical Data
I0800-14S
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:10
Western Blot: 1:40 detects a singe band at ~130kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2b,k7C204Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Recombinant human iNOS
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes human iNOS (aa25-54) and recombinant human iNOS (Type II). Does not react to human nNOS (aa201-231), recombinant human nNOS (Type I), human eNOS (aa25-54) and recombinant human eNOS (Type III). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of human iNOS and was found to bind to a defined region of the human iNOS sequence, residues 25-54.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.