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Technical Data |
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I1000-01A |
Interferon gamma, Recombinant, Mouse (IFNg) |
100ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Recombinant mouse IFN-gamma is a single non-glycosylated polypeptide chain containing 134aa and having a molecular weight of 15.6kD. The sequence of the first five N-terminal amino acids was determined to be Met-His-Gly-Thr-Val. Source: E. coli Molecular Weight: ~15.6kD Biological Activity: Specific activity as determined by the cytopathic affect inhibition assay with mouse NIH 3T3 cells challenged with EMC virus was <0.1ng/ml, corresponding to a specific activity of 1x10e7Units/mg. Endotoxin: 0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O, 0.1% HSA or BSA. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~15.6kD |
Source: E. coli Purity: 95% pure (RP-HPLC and SDS-PAGE). Form: Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute using sterile deionized water to a concentration 100ug/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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