		Technical Data
I1890-60	IgA (AP)

Description:
Label: Alkaline Phosphatase, Calf Intestine. Molecular Weight 140kD

Applications: Suitable for use in ELISA, Western Blotting, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:2000 to 1:4000. Assayed against 1ug of mouse IgG in a standard capture ELISA using pNPP as a substrate for 30 minutes at RT. Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		4°C Do Not Freeze	Blue Ice	Mouse	Rabbit

Concentration:

~1mg/ml

Immunogen:

Mouse IgA heavy chain

Purity:

Purified by mmunoaffinity chromatography using mouse IgA coupled to agarose beads.

Form

Supplied as a liquid in 0.05M Tris HCl , 0.15M sodium chloride, 0.001M magnesium chloride, 0.0001M zinc chloride, 10mg/ml BSA (IgG and Protease free), pH 8.0, 0.01% sodium azide, 50% glycerol. Conjugated to Alkaline Phosphatase.

Specificity:

Single precipitin arc against anti-phosphatase, anti-rabbit serum, mouse IgA and mouse serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse heavy and light chain isotypes.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.