Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilutions:
ELISA: 1:1000-1:10,000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Freezing alkaline phosphatase conjugates will result in a substantial loss of activity. Alkaline phosphatase conjugates are sensitive to light.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ug | | 4°C Do Not Freeze | Blue Ice | Human | Goat |
|
| Concentration: |
| ~0.5mg/ml |
| Immunogen: |
| Native human IgE |
| Purity: |
| Purified by affinity chromatography. |
| Form |
| Supplied as a liquid in 50mM HEPES, 0.1M sodium chloride, 1mM magnesium chloride, 0.1mM ZnCl2, 0.2% BSA, 0.09% sodium azide and. Labeled with alkaline phosphatase (AP). |
| Specificity: |
| Recognizes human IgE |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Khan, F.M. et al. (2012) Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E.
Allergy Asthma Clin Immunol. 8: 1. 2. Pinot de Moira, A. (2012) Childhood schistosome and hookworm co-infections: effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection. Infect Immun. Oct 15.
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