Technical Data
I1900-30A
IgE (HRP)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Affinity Constant:
4 x 10e9 M-1

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG13H2277Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat IgE myeloma protein (IR162, IR410).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity:
Recognizes rat epsilon heavy chain of immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Negrao-Cooea, D. et al. (1996) Intestinal transport and catabolism of IgE: a major blood-independent pathway of IgE dissemination during a Trichinella spiralis infection of rats. J. Immunol. 157: 4037-4044. 2. Bazin, H.et al. (1974) Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours. Immunology 26: 713-723. 3. Bazin, H. et al. (1978) Transplantable IgD immunoglobulin-secreting tumors in rat. J. Immunol. 121: 2077-2082. 4. Bazin, H. et al. (1984) Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum. J. Immunol. Methods. 71: 9-16. 5. Bazin, H. et al. (1984) Rat monoclonal antibodies. I. Rapid purification from in vitro culture supernatants. J. Imm. Methods 66: 261-269.