Description:
Applications:
Suitable for use in ELISA, Immunoelectrophoresis and Electron Microscopy and Western Blot. Other applications not tested.
Recommended Dilutions:
ELISA: 1:20-1:40
Immunoelectrophoresis: 1:20-1:40
Electron Microscopy: 1:20-1:40
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. DO NOT FREEZE!. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are light sensitive.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 300ul | | 4°C Do Not Freeze | Blue Ice | Chicken | Donkey |
|
| Concentration: |
| Not Determined |
| Immunogen: |
| Chicken IgY |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in 0.01M sodium borate/sodium phosphate, 0.15M sodium chloride, pH 8.5, 15 mg/ml BSA (IgG-Free, Protease-Free), 0.05% sodium azide. Labeled with 12nm collidial gold. OD(520) = 2.0. |
| Specificity: |
| Recognizes the whole chicken IgY molecule. Also recognizes with the light chains of other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Minimal crossreactivity to bovine, goat, guinea pig, Syrian hamster, equine, human, mouse, rabbit, rat, sheep serum proteins. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Faulk, W.P. and Taylor, G.M. 1971. An Immunocolloid, Method for the Electron Microscope. Immunochemistry. 8: 1081-1083. 2. Chandler, J., Gurmin, T., and Robinson, N. 2000. The Place of Gold in Rapid Tests. IVD Technology 6, no. 2: 37-49. 3. Hayat, M.A. ed. 1989. Colloidal Gold: Principles, Methods, and Applications, vol. 1. San Diego: Academic Press. 4. Handley, D.A. 1989. Methods for Synthesis of Colloidal Gold. page 22 in Hayat, M.A., ed. Colloidal Gold: Principles, Methods, and Applications. San Diego: Academic Press. 5. Frens, G. 1973. Controlled Nucleation for the Regulation of Particle Size in Monodisperse Gold Solutions. Nature Physical Science. 20 :241.
|
|
