Technical Data
IgG, IgA, IgM, H&L
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
1mg-20CBlue IceRatGoat
~2mg/ml (UV280)
Rat IgG, IgA and IgM whole molecules
Purified by immunoaffinity chromatography followed by solid phase adsorption(s) to remove any unwanted reactivities. A
Supplied as a liquid in 0.1 M Sodium Borate, 0.005 M EDTA, 0.25 M Sodium Chloride, pH 8.2, 0.01% sodium azide
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat immunoglobulin subclasses and chain combinations.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.