		Technical Data
I1904-48J	IgG, IgA, IgM, H&L

Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA : 1:20,000 - 1:100,000
Western Blot : 1:2,000 - 1:10,000
Immunohistochemistry: 1:1,000 - 1:5,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
1mg		-20°C	Blue Ice	Rat	Goat

Concentration:

~2mg/ml (UV280)

Immunogen:

Rat IgG, IgA and IgM whole molecules

Purity:

Purified by immunoaffinity chromatography followed by solid phase adsorption(s) to remove any unwanted reactivities. A

Form

Supplied as a liquid in 0.1 M Sodium Borate, 0.005 M EDTA, 0.25 M Sodium Chloride, pH 8.2, 0.01% sodium azide

Specificity:

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG, Rat IgA and Rat IgM. This reagent is suitable for the detection of all Rat immunoglobulin subclasses and chain combinations.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.