Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Recommended Dilution:
ELISA: 1:1500-1:6000
Western Blot: 1:500- 1:2500.
Immunohistochemistry: 1: 200-1:1000.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Stord at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 1mg | | 4°C Do Not Freeze | Blue Ice | Mouse | Rabbit |
|
| Concentration: |
| ~1mg/ml (UV280) |
| Immunogen: |
| Mouse IgG (gamma heavy chain). MW:140kD. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP). |
| Specificity: |
| Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
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