Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Western Blot: 1:500- 1:2500.
Immunohistochemistry: 1: 200-1:1000.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Stord at 4°C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
|1mg||4°C Do Not Freeze||Blue Ice||Mouse||Rabbit|
| ~1mg/ml (UV280)|
|Mouse IgG (gamma heavy chain). MW:140kD.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).|
|Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|