Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.
Western Blot: 1:500- 1:2500.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
|1mg||4°C Do Not Freeze||Blue Ice||Mouse||Rabbit|
| ~1mg/ml |
|Mouse IgG (gamma heavy chain). MW:140kD.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in 0.05M Tris Chloride, 0.15M sodium chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, pH 8.0, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide, 50% gycerol. Labeled with Alkaline Phosphatase (AP).|
|Recognizes mouse IgG gamma heavy chain. Assay by IEP resulted in a single precipitin arc against anti-alkaline phosphatase (calf intestine) and anti-rabbit serum. Specificity was confirmed by ELISA at <1% crossreactivity against other mouse or human heavy or light chain isotypes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|