Technical Data
I1904-65P
IgG (AP)
Description:
Applications:
Suitable for use in Western Blot, Dot Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 1:1500-1:6000
Western Blot: 1:500- 1:2500.
Immunohistochemistry: 1: 200-1:1000.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Stord at 4C. Do not freeze. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg4C Do Not FreezeBlue IceMouseRabbit
Concentration:
~1mg/ml (UV280)
Immunogen:
Mouse IgG (gamma heavy chain). MW:140kD.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, 1mM magnesium chloride, 0.1mM zinc chloride, pH 8.0, 10mg/ml BSA (IgG and protease free), 0.01% sodium azide, 50% glycerol. Labeled with Alkaline Phosphatase (AP).
Specificity:
Assay by IEP resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine) and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% crossreactivity against other mouse or human heavy or light chain isotypes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.