Technical Data
IgG, Kappa (Biotin)
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)

Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

Application(s): immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Recommended Dilution(s): This product has been assayed against 1ug of Mouse Kappa in a standard capture ELISA using Peroxidase Conjugated Streptavidin. A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.

Storage Conditions: Store vial at 4 C prior to restoration. Restore with 1.0 ml of deionized water (or equivalent). For extended storage reconstitute product with 50% glycerol instead of water and then aliquot contents and freeze at-20 C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
PabIgGAffinity Purified
1mg-20CBlue IceMouseRabbit
1.0 mg/ml (UV280)
Mouse Kappa
Prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Biotin conjugated. Lyophilized in 0.02 M Potassium Phosphate, 0.15M sodium chloride, pH 7.2.Stabilizer: 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-Rabbit Serum. Specificity was confirmed by ELISA at less than 1% against other Mouse heavy or light chain proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.