Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA and Immunodiffusion. Other applications not tested.
Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal working dilutions to be determined by researcher.
Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo
Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 AG14 mouse myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 3H2314 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 200ug | | 4°C (-20°C Glycerol) | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Purified Human IgG1 |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, 0.09% sodium azide. |
| Specificity: |
| Recognizes human IgG1 at an epitope localized to the Fc region. It is negative on IgG2, IgG3, IgG4, free kappa or free lambda, IgM, IgA1 (lambda), IgA2 (kappa) and IgD. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Jefferis, R. et al . (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG subclasses. Immunology Letters. 10: 223-252. Results of an IUIS/WHO collaborative study. 2. Jefferis, R. et al . (1988). Immunology. 63: 171. 3. Jefferis, R. and Kumararatne, D.S. (1990). Selective IgG subclass deficiency quantification and clinical relavance. Clin. Exp. Immunol. 81: 357-367. 4. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of Human IgG. J. Immunol. Methods. 93: 89-96.
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