Technical Data
I1904-76J1
IgG1, Fc
Description:
Applications:
Suitable for use in Immunohistochemistry, ELISA and Immunodiffusion. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0 AG14 mouse myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a3H2314Affinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified Human IgG1
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human IgG1 at an epitope localized to the Fc region. It is negative on IgG2, IgG3, IgG4, free kappa or free lambda, IgM, IgA1 (lambda), IgA2 (kappa) and IgD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jefferis, R. et al . (1985). Evaluation of Monoclonal Antibodies having specificity for Human IgG subclasses. Immunology Letters. 10: 223-252. Results of an IUIS/WHO collaborative study. 2. Jefferis, R. et al . (1988). Immunology. 63: 171. 3. Jefferis, R. and Kumararatne, D.S. (1990). Selective IgG subclass deficiency quantification and clinical relavance. Clin. Exp. Immunol. 81: 357-367. 4. Hussain, R. et al . (1986). Use of monoclonal antibodies to quantify subclasses of Human IgG. J. Immunol. Methods. 93: 89-96.