Technical Data
I1904-80L
IgG2
Description:
IgG is a glycoprotein that is produced by plasma cells in response to an immunogen (1). It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region (1). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide connectivity at the hinge region (2). IgG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab domains and hinge region (3). IgG2-B is a structure defined by a symmetrical arrangement of a (C(H)1-C(L)-hinge)(2) complex with both Fab regions covalently linked to the hinge (3). IgG2-A/B represents an intermediate form, defined by an asymmetrical arrangement involving one Fab arm covalently linked to the hinge through disulfide bonds (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000-1:20,000
Immunohistochemistry: 1:500-1:1000
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
Mab10J104Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in human IgG2.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human IgG2 at ~52kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. G Mayer. Immunoglobulin - Structure and Function. University of South Carolina School of Medicine. 2000 2. Dillon TM, et al. J Biol Chem 283(23):16206-15, 2008 3. Wypych J, et al. J Biol Chem 283(23):16194-205, 2008