Technical Data
I1904-80L
IgG2
Description:
IgG is a glycoprotein that is produced by plasma cells in response to an immunogen (1). It is the only class of immunoglobulins that can cross the placenta, which is mediated by a receptor on placental cells in the Fc region (1). Human IgG2 subclass has been identified to exist as an ensemble of distinct isoforms, designated IgG2-A, -B, and -A/B, which differ by the disulfide connectivity at the hinge region (2). IgG2-A is the known classic structure for the IgG2 subclass defined by structurally independent Fab domains and hinge region (3). IgG2-B is a structure defined by a symmetrical arrangement of a (C(H)1-C(L)-hinge)(2) complex with both Fab regions covalently linked to the hinge (3). IgG2-A/B represents an intermediate form, defined by an asymmetrical arrangement involving one Fab arm covalently linked to the hinge through disulfide bonds (3).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10,000-1:20,000
Immunohistochemistry: 1:500-1:1000
Immunocytochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
Mab10J104Supernatant
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in human IgG2.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human IgG2 at ~52kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. G Mayer. Immunoglobulin - Structure and Function. University of South Carolina School of Medicine. 2000 2. Dillon TM, et al. J Biol Chem 283(23):16206-15, 2008 3. Wypych J, et al. J Biol Chem 283(23):16194-205, 2008