Technical Data
I1904-82P1
IgG2a, Heavy Chain (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 5ug/ml. Use 10ul of the optimal working dilution to label 10e6 cells in 100ul.
Optimal working dilutions to be determined by researcher.

Fusion Partners:
Spleen cells from immunized BALB/c mice were fused with cells of the PAI-0 mouse myeloma cell line.

Recommended Negative Controls:
I1904-78X: IgG1 Murine Negative Control (For rat tissue) (FITC)

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2362Affinity Purified
SizeStorageShippingSourceHost
500ug4C (-20C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Purified rat IgG2a from the IR33-anti-horse ferritin antibody.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, 50% glycerol. Labeled with FITC.
Specificity:
Recognizes the rat IgG2a heavy chain. Does not crossreact with other rat immunoglobulin classes or subclasses.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazin, H., Beckers, A., Querinjean, P., Eur. J. Immunol. 4: 44-48 (1974). 2. Kohler, G., Milstein, C., Nature 256: 495-497 (1975). 3. Schulman, M., Wilde, C.D., Kohler, G., Nature 276: 269-270 (1978). 4. Bazin, H., Beckers, A., Deckers, C., Moriame, M., J. Natl. Cancer Institute 51: 1359-1361 (1973). 5. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Platteeu, B., Urbain, J. J. Immunol. 121: 2077-2082 (1978).