Technical Data
I1904-86G
IgG3, Hinge Region
Description:
Applications:
Suitable for use in ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0-Ag14/X myeloma cells with spleen cells from Balb/c mice.

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo
I1903-13A: IgG, F(ab)2, X-Adsorbed (PE) Pab GtxMo

Recommended Negative Control:
I1904-78R: IgG1 Murine Negative Control

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2421Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Pool of 5-6 individually purified human IgG3 myeloma proteins
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in TBS, 0.09% sodium azide.
Specificity:
Recognizes human IgG3, binding to an epitope at the hinge region. Does not crossreact with IgG1, IgG3, IgG4, free kappa, free lambda, IgM, IgA1, IgA2 or IgD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jefferis, R. et al. (1985). Immunol. Lett. 10: 223-252. 2. Jefferis R et al (1980). Immunol 81: 357-367. 3. Mutapi, F. et al. (2002) The effect of treatment on the age-antibody relationship in children infected with Schistosoma mansoni and Schistosoma haematobium. Mem Inst Oswaldo Cruz. 97 Suppl 1: 173-80.