		Technical Data
I1904-89D	IgG4 (HRP)

Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:25,600-1:51,200
Immunohistochemistry (Frozen sections): 1:40-1:80. Can also be used with paraffin sections.
Optimal dilutions to be determined by the researcher.

IHC Positive Control:
Tonsil

Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1	3H2426	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.2, 0.05% ProclinTM 300, 0.002% Methylisothiazolone, 0.002% Bromonitrodioxane. Labeled with HRP.

Specificity:

Recognizes an epitope in the pFc region of human IgG4. No crossreactivity is observed with human IgG1, IgG2 or IgG3 isotypes. Species crossreactivity: chimpanzee

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Jefferis, R. et al. (1985) Immunology Letters. 10: 223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34: 271-8. 3. Strehl, J.D. et al. (2011) . J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology.72: 94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9: 29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46: 195-8.