Technical Data
I1904-89D
IgG4 (HRP)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:25,600-1:51,200
Immunohistochemistry: Pariffin
Immunohistochemistry (Frozen sections): 1:40-1:80.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Tonsil

Hybrdoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
MabIgG13H2426Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified IgG4
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.05% ProclinTM 300, 0.002% Methylisothiazolone, 0.002% Bromonitrodioxane. Labeled with HRP.
Specificity:
Recognizes an epitope in the pFc region of human IgG4. No crossreactivity is observed with human IgG1, IgG2, IgG3, IgM or IgA isotypes. Species Crossreactivity: chimpanzee
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jefferis, R. et al. (1985) Immunology Letters. 10: 223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34: 271-8. 3. Strehl, J.D. et al. (2011) . J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology.72: 94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9: 29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46: 195-8.