Technical Data
I1904-89D
IgG4 (HRP)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:25,600-1:51,200
Immunohistochemistry (Frozen sections): 1:40-1:80. Can also be used with paraffin sections.
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Positive Control:
Tonsil

Hybrdoma:
Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG13H2426Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified IgG4
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.05% ProclinTM 300, 0.002% Methylisothiazolone, 0.002% Bromonitrodioxane. Labeled with HRP.
Specificity:
Recognizes an epitope in the pFc region of human IgG4. No crossreactivity is observed with human IgG1, IgG2, IgG3, IgM or IgA isotypes. Species Crossreactivity: chimpanzee
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jefferis, R. et al. (1985) Immunology Letters. 10: 223-252. 2. Agaimy, A. et al. (2010) Am J Surg Pathol. 34: 271-8. 3. Strehl, J.D. et al. (2011) . J Clin Pathol. Jan 12. 4. Black, C.M. et al. (1991) Immunology.72: 94-8. 5. Fernandez-Becerra, C. (2010) Malar J. 9: 29. 6. Lee, H.W. et al. (2008) Korean J Parasitol. 46: 195-8.