Suitable for use in Flow Cytometry, Immunoprecipitation, ELISA. Other applications not tested.
Flow Cytometry: 10ul labels 10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Purified Peking duck yolk IgY|
|Purified by Protein G affinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.|
|Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev. and Comp. Immunol. 29: 733-748.|
2. Schneider, K. et al. (2004) Chicken BAFF – a highly conserved cytokine that mediates B cell survival. Int. Immunol. 16: 139-148.