Description:
Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, ELISA. Other applications not tested.
Recommended Dilution: Flow Cytometry: 10ul labels 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Type | Isotype | Clone | Grade |
| Mab | IgG | 7E137 | Affinity purified |
|
| Size | | Storage | Shipping | Source | Host |
| 250ug | | | Blue Ice | Duck | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Purified Peking duck yolk IgY |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. |
| Specificity: |
| Recognizes the light chain of duck immunoglobulin, shown to be expressed strongly on bursal cells and weakly on splenocytes and peripheral lymphoid cells, but not expressed by thymocytes. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Kothlow, S. et al. (2005) Characterization of duck leucocytes by monoclonal antibodies. Dev. and Comp. Immunol. 29: 733-748. 2. Schneider, K. et al. (2004) Chicken BAFF – a highly conserved cytokine that mediates B cell survival. Int. Immunol. 16: 139-148. |
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