Technical Data
I7570-31
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. Inducible NOS (iNOS) or macrophage NOS (mNOS) is a calcium/calmodulin independent and is expressed in activated macrophages and stimulated glial cells.

Applications:
Sutiable for use in Immunohistchemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin/paraffin): Ab at 1:10-1:25 for 30 min. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control: Lung

Epitope: C-terminus

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul4°C (-20°C Glycerol)Blue IceMouseRabbit
Concentration:
~0.1mg/ml
Immunogen:
A synthetic peptide derived from C-ternimal of mouse iNOS. Cellular Localization: Cytoplasmic
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.6, 0.2% BSA and 15mM sodium azide. before the addition of glycerol to 40%.
Specificity:
Recognizes human iNOS. Species Crossreactivity: Rat and mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.