Technical Data
I7649-97B
Influenza A Neuraminidase NA (New Jersey 11/76, H1N1)
Description:
Influenza A H1N1 NA, also known as Neuraminidase, is a protein belonging to the Influenza A virus strain (strain A/Swine/New Jersey/11/1976 H1N1) with 469aa. It belongs to the glycosyl hydrolase 34 family. This Influenza A virus has aves, human and pig as its host. Neuraminidase is a homotetramer located in the virion membrane and host apical cell membrane and preferentially accumulates at the apical plasma membrane in infected polarized epithelial cells, which is the virus assembly site. This protein is a receptor-destroying enzyme that catalyzes the removal of terminal sialic acid residues from viral and cellular glycoconjugates (cellular receptors) during virus budding to facilitate virus release, spreading, prevents self-aggregation of virus and ensures the efficient spread of the progeny virus from cell to cell. It may facilitate viral invasion of the upper airways by cleaving the sialic acid moieties on the mucin of the airway epithelial cells. Neuraminidase plays a role in the determination of host range restriction on replication and virulence.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa35-48 (SHSIQTGEKSHPKV) of Influenza A H1N1 NA (swine flu).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes Influenza A H1N1 NA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Moscona A. N. Engl. J. Med. 353:1363-1373 (2005). 2. Suzuki Y. Biol. Pharm. Bull. 28:399-408 (2005). 3. Nayak D. P. et al. Virus Res. 106:147-165 (2004).