Technical Data
ING1, p33 (Inhibitor of Growth 1)
The ING1 (inhibitor of growth) tumor suppressor protein shares a number of biological functions that are very similar with p53. The human ING1 gene contains three exons and two introns. There are four alternative splice variants produced from three different promoter regions (p33 ING1, p24 ING1, p27 ING1, p47 ING1). A number of studies have concluded that ING1 can influence a number of biological functions, which might be dependent on the variant that is expressed. The ING1 family has been reported to mediate growth arrest, senescence, anchorage-dependent growth and apoptosis, as well as influence chemosensitivity. (1-4).

Suitable for use in Western Blot, Immunocytochemistry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot (Colorimetric): 1ug/ml
Immunocytochemistry: 25ug/ml
ELISA: 1-2ug/ml

Optimal dilutions to be determined by researcher.

Positive Controls:
Mouse Brain Tissue Extract

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug4C (-20C Glycerol)Blue IceMouseRabbit
Mouse p33 ING1 N-terminal peptide, KLH conjugated.
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, 0.05% sodium azide.
Detects an ~33kD protein, corresponding to the apparent MW of p33 ING1 on SDS-PAGE Immunoblots in samples of mouse and human origin. Does not cross react with p24 ING or p47 ING.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Cheung, K.J., Jr. and Li, G. (2002) Int. J. Oncol. 21(6): 1361-1365. 2. Cheung, K.J., Jr. and Li, G. (2002) Exp. Cell Res. 279(2): 291-28. 3. Cheung, K.J., Jr. and Li, G. (2002) Int. J.Oncol. 20(6):1319-1322. 4. Cheung, K.J., Jr. and Li, G. (2001) Exp. Cell Res. 268(1): 1-6.