Technical Data
Insig1 (Insulin-induced Gene 1 Protein, INSIG-1)
Oxysterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element-binding protein (SREBP) mediated signaling pathway. This gene is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. This protein binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Membrane

Positive Control: HepG2 lysate

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabAffinity Purified
100ul-20CBlue IceHumanRabbit
A synthetic peptide representing an internal region of the human protein (between residues 1-100) according to Swiss-Prot# O15503. Homology: 100% with human protein. No other significant species homology.
Purified by immunoaffinity chromatography.
PBS + 30% glycerol
This antibody is specific for Insig1 but not Insig2. Species Crossreactivity: This antibody reacts with human protein. Other species have not been tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Engelking, LJ, et al. J. Clin. Invest. 115(9): 2489-2498 (2005)
2. Gong, Y., Cell Metabolism. 3: 15-24 (2006)