Technical Data
I7660-11
Insulin
Description:
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Use as conjugate in ELISA sandwich assay with clone 0.BG.22 as solid phase. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen
To be determined by the researcher.

Recommended Pair:
Suitable for use as the detection or conjugate (label) antibody.
Matched to I7660-10 as the capture or solid phase antibody.

Affinity Constant:
1.5 x 10e8 L/mole

Hybridoma:
X63-Ag8-653 myeloma cells with spleen cells of BALB/c mice.

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommended adding glycerol as a cryopreservative.
TypeIsotypeCloneGrade
MabIgG10.BG.23Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Purified human Insulin
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Specificity:
Recognizes human Insulin. Minimal reactivity with other serum proteins. Species Crossreactivity: Bovine and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.