Technical Data
Insulin is one of the major regulatory hormones of intermediate metabolism throughout the body. The biological actions of this hormone involve integration of carbohydrate, protein, and lipid metabolism. Insulin enhances membrane transport of glucose, amino acids and certain ions. It also promotes glycogen storage, formation of triglycerides and synthesis of proteins and nucleic acids. Immunocytochemical investigations have localized insulin in the B-cells of pancreatic islets of Langerhans. Deficiency of insulin results in diabetes mellitus, one of the leading causes of morbidity and mortality in the general population. Insulin is also present in tumors of B-cell origin such as insulinoma.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen/paraffin): 1:100-1:500
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20CBlue IceGuinea pig
Not Determined
Supplied as a liquid in PBS, pH 7.4, protein base, <0.1% sodium azide.
Recognizes Insulin. Staining pattern: Cytoplasmic.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Akagi, T, et al. Cancer 1981;47:417-424 2. Scully, RE, et al. N eng J Med 1983;308:30-37 3. Erlandsen, SL, Williams and Wilkins, Baltimore, 1980 pp140-155 4. Friesen, SR, N Eng J Med 1982;306:580-590 5. Jorda M et al. Arch Pathol Lab Med. 2003 Feb;127(2):196-9 6. Letizia C et al. Eur J Endocrinol. 2001 May;144(5):517-20 7. Govindarajan M et al. Diabetes Res Clin Pract. 2001 Jan;51(1):29-38 8. Azzoni C et al. Virchows Arch. 1998 Dec;433(6):495-504 9. Lubensky IA et al. Am J Pathol. 1998 Jul;153(1):223-31.