Technical Data
I7660-22
Insulin
Description:
Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Hybridoma:
Hybridization of X63-Ag8-653 myeloma cells with spleen cells of Balb/c mice immunized with purified human insulin.

Reactiviy:
Kass is 7.5 x 10e9Me-1

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k5C54Affinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~5mg/ml
Immunogen:
Purified human insulin
Purity:
Purified by Protein A affinity chromatography. Purity is tested by electrophoresis.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% of sodium azide. No glycerol added
Specificity:
Recognizes human Insulin. Reacts with C-terminal pentapeptide of insulin beta-chain. Crossreact with human proinsulin, bovine insulin (30%) and porcine insulin. No crossreaction with free C-peptide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Effects of insulin-dependent diabetes mellitus on perforator-based flaps in streptozotocin diabetic rats. Serdaroglu I, Islamoglu K, J Reconstr Microsurg 2005 Jan;21(1):51-6 2. Retinal neovascularization during treatment with IGF-1 for insulin resistance syndrome. Kitamei H, Yokoi M, Ohno S, Graefes Arch Clin Exp Ophthalmol 2005 Jan 26; 3. Childhood obesity and insulin resistance in a Yucatan mini-piglet model: putative roles of IGF-1 and muscle PPARs in adipose tissue activity and development. Sebert SP, Lecannu G, Siliart B, Int J Obes Relat Metab Disord 2005 Jan 11; 4. Insulin determines leptin responses during a glucose challenge in fed and fasted rats. Walker CG, Bryson JM, Hancock DP, Int J Obes Relat Metab Disord 2005 Jan 11; 5. Insulin and glyburide therapy: Dosage, severity level of gestational diabetes, and pregnancy outcome. Langer O, Yogev Y, Rosenn B, Am J Obstet Gynecol 2005 Jan;192(1):134-9 6. What are capillary blood ketone levels in type 1 diabetic patients using CSII in normal conditions of insulin delivery? Matta M, Melki V, Hanaire-Broutin H, Diabetes Metab 2004 Dec;30(6):543-7.