Technical Data
I7660-90
Insulin-Like Growth Factor I E3R, IGFBP1 Resistant, 70aa, Recombinant, Human (IGF1)
25ug
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Technical Data |
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I7660-90 |
Insulin-Like Growth Factor I E3R, IGFBP1 Resistant, 70aa, Recombinant, Human (IGF1) |
25ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Recombinant human IGF-I (E3R) expressed in E.coli. IGF-I (E3R) (insulin-like growth factor I) (resistant to IGFBPs) is a 70 amino acid analog of human IGF-I with the substitution of an Arg for Glu at position 3. IGF-I (E3R) is signi cantly more potent than IGF-I in vitro and in vivo because IGF-I (E3R) has decreased binding to all IGF binding proteins. Biological Activity: Stimulation of protein synthesis in L6 myoblasts: ED50 = 0.9ng/ml. This lot at 5ng/ml stimulated incorporation of [3H] thymidine in chick embryo broblast cells 1.6-fold over background. Rehydration: In 100mM acetic acid or 10mM HCl. When preparing solutions at concentrations of 1mg/ml add a carrier protein to minimize adsorption of peptide to plastic or glass surfaces. Use RIA grade bovine serum albumin at concentrations between 0.1-1mg/ml. For use in the presence of fetal bovine serum in cell culture, no carrier protein is necessary. Molecular Weight: 7.6kD |
Source: E. coli Purity: 95% by HPLC. Puri ed by liquid chromatography. Filtered through a 0.2um membrane and packaged aseptically. Concentration: ~0.25mg/ml Form: Lyophilized from 100ul 0.1M acetic acid. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Lowe, W.L., In: Insulin-Like Growth Factors: Molecular and Cellular Aspects LeRoith, D., CRC Press Inc. Boca Raton, p49, 1991. 2. LeRoith, D., and C.T. Roberts Jr., Ann. New York Acad. Sci. 692: 1, 1993. 3. Francis, G.L., J. Mol. Endocrinol. 8: 213–223, 1992.
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