Technical Data
Insulin-Like Growth Factor I E3R, IGFBP1 Resistant, 70aa, Recombinant, Human (IGF1)
Molecular Biology Storage: -20CShipping: RT
Recombinant human IGF-I (E3R) expressed in E.coli. IGF-I (E3R) (insulin-like growth factor I) (resistant to IGFBPs) is a 70 amino acid analog of human IGF-I with the substitution of an Arg for Glu at position 3. IGF-I (E3R) is signi cantly more potent than IGF-I in vitro and in vivo because IGF-I (E3R) has decreased binding to all IGF binding proteins.

Biological Activity: Stimulation of protein synthesis in L6 myoblasts: ED50 = 0.9ng/ml. This lot at 5ng/ml stimulated incorporation of [3H] thymidine in chick embryo broblast cells 1.6-fold over background.

Rehydration: In 100mM acetic acid or 10mM HCl. When preparing solutions at concentrations of 1mg/ml add a carrier protein to minimize adsorption of peptide to plastic or glass surfaces. Use RIA grade bovine serum albumin at concentrations between 0.1-1mg/ml. For use in the presence of fetal bovine serum in cell culture, no carrier protein is necessary.

Molecular Weight:
Source: E. coli
Purity: 95% by HPLC. Puri ed by liquid chromatography. Filtered through a 0.2um membrane and packaged aseptically.
Concentration: ~0.25mg/ml
Form: Lyophilized from 100ul 0.1M acetic acid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Lowe, W.L., In: Insulin-Like Growth Factors: Molecular and Cellular Aspects LeRoith, D., CRC Press Inc. Boca Raton, p49, 1991. 2. LeRoith, D., and C.T. Roberts Jr., Ann. New York Acad. Sci. 692: 1, 1993. 3. Francis, G.L., J. Mol. Endocrinol. 8: 213223, 1992.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.