Technical Data
I7661-05
Insulin-Like Growth Factor I (IGF1, Somatomedin C)
Description:
Insulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges. IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/ autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5-2ug/ml detects 100ng of IGF1 (Cat. #I7661-11A) under non-reducing conditions.
Immunoprecipitation: 5ug immunoprecipitated 100ng IGF1.
Neutralization: 10-20ug/ml can inhibit the activity of 10ng/ml of IGF1 as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay.
Immunohistochemistry: 10ug/ml. Reported to detect IFG1 in formalin-fixed, paraffin-embedded skin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k0.T.61Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Human insulin-like growth factor I (IGF1) purified from human plasma.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from 0.1M Tris-glycine, pH 7.4, 0.15mM sodium chloride, 0.1mM EDTA. No preservative added. Reconstitute with 200ul sterile ddH2O.
Specificity:
Recognizes human Insulin-Like Growth Factor I (IGF1), Mr ~17kD (migrates to ~7kD). Crossreactivity with IGF-II is ~40%. Species Crossreactivity: mouse, rat and possibly chicken. Occasional difficulty neutralizing rat IGF1 due to binding protein interference.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Harvey, A., et al., J. BIol. Chem. 274: 23,249-23,255 (1999). 2. Manes, S., et al., J. Biol. Chem. 274: 6935-6945 (1999). 3. Russell, W.E., et al., Proc. Natl. Acad. Sci. USA. 81: 2389-2392 (1984). 4. van Wyk, J.J., et al., Human Growth Hormone 585-599, Plenum Publishing Corp, NY (1986). 5. van Wyk, J.J., et al., Endocrinology 138: 4521 (1997). 6. Liu, et al., Molc. Cell. Neurosci. 5: 418-429 (1994).