Technical Data
Insulin-Like Growth Factor I, Recombinant, Mouse (IGF1)
Molecular Biology Storage: -20CShipping: RT
A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.

Recombinant murine IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7.6kD.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids was determined and was found to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically.

Biological Activity:
IGF-I is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells (measured by 3H-thymidine uptake) is 1.0ng/ml, corresponding to a specific activity of 1x10e5 U/mg.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: Recombinant corresponding to murine expressed in (E. col)
Purity: 98% by RP-HPLC, SDS-PAGE.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives. Reconstitute with ddH2O, 0.1% HSA or BSA to 100ug/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Inhibitory effect of IGF-I on induced apoptosis in mouse preimplantation embryos cultured in vitro. Fabian D, Il'kova G, Czikkova S, Theriogenology 2004 Feb;61(4):745-55 2. Insulin-like growth factor-I governs submucosal growth and thickness in the newborn mouse ileum. Herman AC, Carlisle EM, Gordon PV, Pediatr Res 2004 Mar;55(3):507-13 3. Evaluation of mouse preimplantation embryos exposed to oxidative stress cultured with insulin-like growth factor I and II, epidermal growth factor, insulin, transferrin and selenium. Kurzawa R, Glabowski W, Brelik P, Reprod Biol 2002 Jul;2(2):143-62 4. Development of leydig cells in the insulin-like growth factor-I (igf-I) knockout mouse: effects of igf-I replacement and gonadotropic stimulation. Wang G, Hardy MP, Biol Reprod 2004 Mar;70(3):632-9 5. Insulin-like growth factor-I improves cerebellar dysfunction but does not prevent cerebellar neurodegeneration in the calcium channel mutant mouse, leaner. Nahm SS, Frank TC, Sepulvado JM, Neurobiol Dis 2003 Nov;14(2):157-65 6. Insulin-like growth factor I (IGF-I) regulates endocrine activity of the embryonic testis in the mouse. Villalpando I, Lopez-Olmos V, J Steroid Biochem Mol Biol 2003 Aug;86(2):151-8.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.