Technical Data
Insulin-Like Growth Factor I, Recombinant, Rat (IGF1)
Molecular Biology Storage: -20CShipping: RT
A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor.

Recombinant Rat IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7687 Dalton.

Biological Activity:
Rat IGF-1 is fully biologically active when compared to standards. The ED50, was calculated using 2 methods:
1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be less then 30ng/ml.
2. Type 1 IGF receptor binding assay ED50 was found to be less then 10ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder in 100mM acetic acid. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration of > 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Kloting, N. et al., (2008) Diabetes 57:2074-2082. 1. Shimatzu, A. & Rotwein, P., J. Biol. Chem. 262: 7894-7900 (1987). 2. Upton, Z., et al., J. Endocrinol. 149: 379-387 (1996).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.