Technical Data
Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)
Molecular Biology Storage: -20CShipping: Blue Ice
IGF-I (E3R) (insulin-like growth factor I) is a 70 amino acid analog of human IGF-I with the substitution of an Arg for Glu at position 3. IGF-I (E3R) is significantly more potent than IGF-I in vitro and in vivo because IGF-I (E3R) has decreased binding to all IGF binding proteins.

Biological Activity:
Stimulation of protein synthesis in L6 myoblasts: ED50 = 19.9ng/ml.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding 100mM acetic acid or 10mM HCL. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. .
Source: E. coli
Purity: >95% by SDS-Page and HPLC.
Form: Supplied as a lyophilized powder form 0.1M acetic acid. Reconstitute in 100mM acetic acid or 10mM HCl. Diluting to concentrations of <1mg/ml requires addition of carrier protein (0.1-mg/ml RIA grade BSA) to minimize adsorption of peptide to glass or plastic surfaces. A carrier protein is not necessary for use in the presence of fetal bovine serum in cell culture.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Bayne, M.L., et al., J. Biol. Chem. 265: 1564815652, 1990. 2. Firth, S.M. & R.C. Baxter, Prog. Growth Factor Res. 6: 223229, 1995. 3. Firth, S.M., et al., J. Biol. Chem. 273: 26312638, 1998.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.