		Technical Data
I7661-11E	Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)	100ug

Growth Factors, Cytokines

Storage: -20°CShipping: Blue Ice

Recombinant Human IGF-1 is a single, non-glycosylated, polypeptide chain containing 70aa and having a molecular weight of 7,655Da. The sequence of the first five N-terminal amino acids was determined to be Gly-Pro-Glu-Thr-Leu. N-terminal methionine has been completely removed enzymatically. Contains less than 1% dimers and aggregates.

Source: E. coli

Molecular Weight: ~7.65kD

Endotoxin: 0.1 ng/ug

Activity:
The ED50 as determined by the dose-dependent proliferation of murine Balb/c 3T3 cells (measured by H-thymidine uptake) is <1.0ng/ml, corresponding to a specific activity of 1x10U/mg. For most in vitro applications, IGF-I exerts its biological activity in the concentration range of 0.2-20ng/ml.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile deionized water to a concentration 100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~7.65kD

Source: E. coli
Purity: 98% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
Form: Supplied as a lyophilized powder from 50mM acetic acid. No preservative added. Reconstitute with sterile deionized water to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.