Technical Data
I7661-21N
Insulin-Like Growth Factor I Receptor, beta (IGF1Rb)
Description:
Type I insulin-like growth factor receptor (IGF1R), a transmembrane receptor tyrosine kinase, is widely expressed in many cell types within fetal and postnatal tissues, and in many cell lines (1-3). Upon binding to its ligands, IGF-I and IGF-II, receptor autophosphorylation occurs. The triple tyrosine cluster within the kinase domain (Tyr1131, Tyr1135 and Tyr1136) is the earliest major site of autophosphorylation (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant similarity with IGF-I receptors in both structure and function. There is also an equivalent triple tyrosine cluster within the activation loop of the kinase domain (Tyr1146, Tyr1150 and Tyr1151). Tyrosine autophosphorylation of insulin receptor is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation of Tyr1146 and either Tyr1150 or Tyr1151. Full kinase activation requires the triple tyrosine phosphorylation (8).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:2400. Use citrate as unmasking buffer.
Immunoprecipitation: 1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to the carboxy-terminal residues of human IGF1Rb.
Purity:
Purified by Protein A and peptide affinity chromatography.
Form
Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of human Insulin-Like Growth Factor I Receptor beta at ~95kD. Does not crossreact with insulin receptor. Species Crossreactivity: mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Adams, T.E., et al., Cell. Mol. Life Sci. 57: 1050–1093 (2000). 2. Baserga, R., et al., Oncogene 19: 5574–5581 (2000). 3. Scheidegger, K.J., et al., J. Biol. Chem. 275: 38,921–38,928 (2000). 4. Hernandez-Sanchez, C., et al., J. Biol. Chem. 270: 29,176–29,181 (1995). 5. Lopaczynski, W., et al., Biochem. Biophys. Res. Commun. 279: 955–960 (2000). 6. Baserga, R., et al., Exp. Cell Res. 253: 1–6 (1999). 7. White, M.F., et al., J. Biol. Chem. 260: 9470–9478 (1985). 8. White, M.F., et al., J. Biol. Chem. 263: 2969–2980 (1988).