Technical Data
I7661-31J
Integrin, alpha1, beta1 (CD49a, VLA alpha1, Laminin Receptor, Collagen Receptor)
Description:
Human alpha 1 integrin sub-unit forms the VLA-1 heterodimer in association with the beta 1 integrin. VLA-1 is a receptor for collagen and laminin, and is expressed by chronically activated T cells, melanoma cells and smooth muscle cells.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:25-1:50. Use 10ul to label 10e6 cells in 100ul.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Recommended Secondary Reagents:
F(ab')2 rabbit anti mouse IgG:RPE
I1904-66A: IgG, F(ab)2 (FITC) Pab Rb xMo
I1904-65W: IgG, F(ab)2 (HRP) Pab Rb xMo
I1904-66C: IgG, F(ab)2 X-adsorbed (HRP) Pab Rb xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A410Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human CD49a
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human alpha 1 integrin subunit, which forms the VLA-1 heterodimer in association with the beta 1 integrin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hemler, M. E. et al. (1984). Glycoproteins of 210,000 and 130,000 m.w. on activated T cells: Cell distribution and antigenic relation to components on resting cell lines. J. Immunol. 132: 3011. 2. Hemler, M. E. et al. (1985). Biochemical characterisation of VLA-1 and VLA-2. J. Biol. Chem. 260: 15246. 3. Hafler, D. A. et al. (1985). Investigation of in vivo activated T cells in multiple sclerosis and inflammatory central nervous system diseases. Clin. Immunol. Immunopathol. 37: 163.