Technical Data
I7661-37E4
Integrin alphaV, beta3
Description:
The involvement of integrins in vascular proliferation, adhesion, and wound repair and have been well-documented. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. (Cancer and Metastasis Reviews 1991. 10:3). Due to its involvement in angiogenesis, the integrin aVb3 receptor is one of the most intensely studied of the integrin receptors.

Applications:
Suitable for use in Immunoprecipitation, Immunoflourescence, Immunohistochemistry and Inhibition. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry/Immunofluorescence: 10ug/ml for staining of bFGF-treated 6um cryosections of chick chorioallantoic membrane fixed with acetone.
Inhibition: 10-25ug/ml adheres cells to vitronectin coated surfaces.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG12Q880Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Integrin alphaV, beta3 from human placenta.
Purity:
Purified by Protein A Sepharose chromatography
Form
Supplied as a liquid in 0.02M PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: bovine, chick, monkey, porcine, canine, rabbit and avian. Not reactive with rat tissue.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.J. Biol. Chemistry 262:17703–17711 (1987). 2.PNAS. USA 84:6471–6475 (1987). 3.J. Cell Biol. 121:1141–1152 (1993). 4.Science 264:569–571 (1994). 5.J. Biol. Chemistry 271:2443–2447 (1996). 6. Cancer Research 59: 1635–1641(1999). 7. Blood 94(2): 649–662 (1999).