Technical Data
I7661-39J
Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla)
Description:
Integrin beta 1 is a 130kD transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Applications:
Suitable for use in Immunoprecipitation, Flow Cytometry, Immunohistochemistry and Western Blot. Not suitable for use in Inhibition. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 2ug; lysate of 10e6 cell
Flow Cytometry: 10ug/ml
Immunohistochemistry (Frozen): 20ug/ml. As a result of the wide tissue distribution of VLA (b1), binding to diverse cell types is commonly observed.
Western Blot: 1:1000 on 10ug C2C12 lysate. C2C12 lysates was resolved by electrophoresis, transferred to PDVF membrane and probed with I7661-39J. Proteins were visualized using an IgG (HRP) goat anti-rat and a chemiluminescent detection system.
Inhibition: Does not block VLA integrin mediated cell adhesion to matrix proteins.
Optimal dilution determined by the researcher.

Positive Controls:
Human OVCAR5 cell line whole cell lysate, C2C12 lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k2Q841Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Mouse Integrin, beta1.
Purity:
Purified by Protein A chromatography.
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes b1 subunit of mouse VLA (b1) integrins at ~88kD, and ~130kD with glycosylation. Species sequence homology: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ballestrom, C.G. et al. (1996) Hybridoma 15: 125-132.