Technical Data
Interferon alpha 4, Recombinant, Mouse (Interferon alpha-4, IFN-alpha-4, Ifna4)
Molecular Biology Storage: -70°CShipping: Dry Ice
Most mammalian species have multiple IFN-a subtypes. Although the reasons for these multiple subtypes are not fully known, there are clear cell type and temporal differences in their expression. A recent study established a nomenclature for the murine IFN-a subtypes (van Pesch et al. 2004) and determined relative activities of the subtypes with protein quantification by phosphorimaging of metabolically-labeled protein. In this study, Mu IFN-a4 was deemed to have average antiviral activity when compared with the potency of the other subtypes. Mu IFN-a4 was initially cloned by Zwarthoff et al. [(1985) Nuc. Acids Res. 13(3) 791], and has been extensively studied. It is apparently expressed early in viral infection in a protein synthesis independent manner, and its expression is induced by phosphorylation of IRF-3. It may be that Mu IFN-a4, like IFN-b, has a priming function on cells, enabling the expression of other Mu IFN-a subtypes [Reviewed by Mesplède et al. (2003) Autoimmunity 36(8):447 and Asselin-Paturel & Trinchieri (2005) J. Exp. Med. 202(4):461]. Thus, this interferon is among the first observed after viral infection. Intriguingly, while this interferon is expressed in a large variety of cell types, one report suggests that the expression level in dendritic cells is low to non-existent [Barchet et al. (2002) J. Exp. Med. 195(4):507]

Recombinant corresponding to murine (C57/Bl) DNA expressed in mammalian cells.

Molecular Weight:

Biological Activity:
Determined relative to a lab standard of Mu IFN-alpha A which was calibrated to the NIH Murine IFN-a standard (Ga02-901-511). Mouse (L929/EMCV) in this assay the EC50 for IFN is ~5U/ml.

Endotoxin: ~1EU/ug

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Molecular Weight:
Source: Mammalian tissue culture media
Purity: ~95% (Ion exchange, hydrophobic interaction and size exclusion chromatography)
Concentration: As reported
Form: Supplied as a liquid in PBS, 0.1% BSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.