Technical Data
Interferon beta, Recombinant, Mouse (CHO) (IFNb)
Molecular Biology Storage: -20CShipping: Blue Ice
Three types of interferons have been described: interferon-alpha (IFN-alpha), interferon-beta (IFN-beta), and interferon-gamma (IFN-gamma). An extensive variety of biological effects have been observed for the interferon family including antiviral activity; anti-proliferative activity; stimulation of cytotoxic activities in lymphocytes, natural killer cells, and macrophages; and modulation of the major histocompatibility complex. Only one mouse-IFN-beta gene has yet been described. It codes for a 161 amino acid polypeptide (20kD). Mouse-IFN-beta binds to the type I interferon receptor. It is not recognized by antibodies directed against mouse
alpha interferon or mouse gamma interferon.

One unit of mouse beta interferon is the amount of beta interferon which protects 50% of the indicator cell population from viral destruction. One unit of Mouse Beta Interferon approximates the bioactivity contained in one unit of the mouse beta interferon standard prepared by NIH (Gb02-902-511).

CHO cells

Recommended Dilution:
For dilutions use a buffer containing 2% bovine serum albumin or culture medium. (Do not use fresh serum.)

Application Notes:
Activity is an average of mulitple determinations. Be aware that activity is dependent on the type of IFN assay and vary between labs. Bioactivity was determined using IFN- sensor cells by monitoring the activation of the JAK/STAT/ISGF3 pathway.

Storage and Stability:
Lyophilized powder may be stored at-20C. Stable for 12 months at -20C. Once reconstituted, store stock solution in aliquots at -20C. Reconstituted product is stable for 6 months at -20C. Note: Mouse IFN-beta is highly labile when agitated. Repeated freeze and thaw cycles and prolonged exposure to light will cause loss of activity.
Source: CHO cells
Purity: Purified
Form: Supplied as a powder lyophilized from a solution containing PBS, 10% mouse albumin and 0.01% gelatin. Reconstitute the vial by injection of 1ml distilled or de-ionized water (Caution: vial is under vacuum).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. De Maeyer, E et al; Interferons and Other Regulatory Cytokines, 1st ed., 1988, Wiley and Sons, New York 2. Vonk, W et al; Large-scale, one-step purification of murine interferon-beta using a monoclonal antibody. J of Interferon Res 1983, 3: 169

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.