Technical Data
I7662-26
Interferon Induced Protein with Tetratricopeptide Repeats 2 (IFIT2, cig42, G10P2, Glucocorticoid Attenuated Response Gene 39, GARG39, Interferon Induced 54kD Protein, IFI 54K, Interferon Induced Protein 54, IFI54, Interferon alpha Inducible Protein (MW 54
Description:
Saha et al. used this antibody to study protein expression of murine IFIT2 in relation to viral infection. Immunofluorescence (IF) localized this protein in association with the mitotic spindle in both mitotically active normal NIH3T3 and B16F10 melanoma cells. Western Blot (WB) shows ~55kD band IFIT2 in brain tissue of mice infected with Japanese encephalitis virus (JEV). Brain tissue from non-infected mice did not display this band. In 1994 Bluyssen et al. characterized the gene for murine IFIT2 and historically the protein has been identified as interferon-induced protein with tetratricopeptide repeats 2 (IFIT2), interferon-induced 54kD protein (IFI-54K), glucocorticoid-attenuated response gene 39 protein (GARG-39). Human IFIT2, with 62% aa sequence identity, has been referred to as Interferon-induced protein with tetratricopeptide repeats 2 (IFIT2), Interferon-induced 54kD protein (IFI-54K) and ISG-54. K. Kang et al. showed with proteome analysis IFIT2 was expressed more than two-fold in human osteosarcoma cells U2OS treated with ascochlorin. Kawada et al. studied gene expression to show that IFIT2 is strongly upregulated in peripheral blood in children during the acute phase of influenza.

Applications:
Suitable for use in Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 25-50ug/ml
Western Blot: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant Interferon Induced Protein with Tetratricopeptide Repeats 2 (IFIT2).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes IFIT2. Species Crossreactivity: mouse and human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wacher, C., et al., J Virol. 81(2): 860-871 (2007). 2. Bluyssen, H.A., et al., Genomics 24(1): 137-148 (1994). 3. Kang, J.H., et al., J. Proteome Res. 5(10): 2620-2631 (2006). 4. Kawada, J., et al., J. Gen. Virol. 87(6): 1677-1683 (2006). 5. Saha, S., et al., Brain Res. 1148: 38-42 (2007). 6. Saha, S., et al., J. Gen. Virol. 87(11): 3285-3289 (2006).