Technical Data
I7663-01
Interleukin 1 alpha, Recombinant, Mouse (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
2ug
10ug
10ug
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Technical Data |
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I7663-01 |
Interleukin 1 alpha, Recombinant, Mouse (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF) |
2ug 10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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IL-1alpha is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Murine IL-1a is a 17.9kD protein containing 156 amino acid residues. Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug Source: E. coli Form: Lyophilized from 1mM Tris buffer solution. May also be supplied as a liquid. Stability: The lyophilized protein, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted murine IL-1a should be stored in working aliquots at -20°C. Reconstitution: Lyophilized murine IL-1a is soluble in water and most aqueous buffers. The lyophilized protein can be reconstituted in water to a concentration of 100ug/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use. For most in vitro applications, IL-1a exerts its biological activity in the concentration range of 0.1 to 10.0 ng/ml. Biological Activity: Murine IL-1a is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of murine D10S cells is 0.002ng/ml, corresponding to a specific activity of 5x10e8 units/mg. Molecular Weight: 17.9kD |
Source: E. coli Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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