Technical Data
I7663-01A1
Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to rat Interleukin 1 alpha expressed in E. coli.

Molecular Weight:
~18kD

Endotoxin: 0.1 ng/ug

Activity:
ED50 range =0.001-0.005 ng/ml, determined by the dose dependent proliferation of mouse D10S cells. The optimal concentration for individual application should be determined by an initial dose response assay.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18kD
Source: E. coli
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Nishida, T., N. Nishino, M. Takano, Y. Sekiguchi, K. Kawai, K. Mizuno, S. Nakai, Y. Masui, and Y. Hirai (1989) Molecular cloning and expression of rat interleukin-1 alpha cDNA. J. Biochem. 105:351-357.2. Orencole, S.F. and C.A. Dinarello (1989) Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin-1 in the absence of mitogens. Cytokine 1:14-22.3. Hirano, T., (1994) In: The Cytokine Handbook, 2nd Edition, (ed A. Thomson), Academic Press Limited, San Diego, Ca, pp. 145-168.4. Dinarello, C. (1996) Biological basis for interleukin-1 in disease. Blood 87:2095-2147. 5. Anforth, H.R., R. Bluthe, A. Bristow, S. Hopkins, M. Lenczowski, G. Luheshi, J. Lundkvist, B. Michaud, Y. Mistry, A. Van Dam, C. Zhen, R. Dantzer, S. Poole, N. Rothwell, F. Tilders, and E. Wollman (1998) Biological activity and brain actions of recombinant rat interleukin-1alpha and interleukin-1beta. Eur. Cytokine Netw. 9:278-288.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.