Technical Data
I7663-01A1
Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF)
10ug
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Technical Data |
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I7663-01A1 |
Interleukin 1 alpha, Recombinant, Rat (IL-1a, Hematopoietin-1, Lymphocyte-activating Factor, LAF, Endogenous Pyrogen, Leukocyte Endogenous Mediator, LEM, Mononuclear Cell Factor, MCF) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to rat Interleukin 1 alpha expressed in E. coli. Molecular Weight: ~18kD Endotoxin: 0.1 ng/ug Activity: ED50 range =0.001-0.005 ng/ml, determined by the dose dependent proliferation of mouse D10S cells. The optimal concentration for individual application should be determined by an initial dose response assay. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~18kD |
Source: E. coli Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Nishida, T., N. Nishino, M. Takano, Y. Sekiguchi, K. Kawai, K. Mizuno, S. Nakai, Y. Masui, and Y. Hirai (1989) Molecular cloning and expression of rat interleukin-1 alpha cDNA. J. Biochem. 105:351-357.2. Orencole, S.F. and C.A. Dinarello (1989) Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin-1 in the absence of mitogens. Cytokine 1:14-22.3. Hirano, T., (1994) In: The Cytokine Handbook, 2nd Edition, (ed A. Thomson), Academic Press Limited, San Diego, Ca, pp. 145-168.4. Dinarello, C. (1996) Biological basis for interleukin-1 in disease. Blood 87:2095-2147. 5. Anforth, H.R., R. Bluthe, A. Bristow, S. Hopkins, M. Lenczowski, G. Luheshi, J. Lundkvist, B. Michaud, Y. Mistry, A. Van Dam, C. Zhen, R. Dantzer, S. Poole, N. Rothwell, F. Tilders, and E. Wollman (1998) Biological activity and brain actions of recombinant rat interleukin-1alpha and interleukin-1beta. Eur. Cytokine Netw. 9:278-288.
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